MP4 | Video: h264, 1280x720 | Audio: AAC, 44.1 KHz
Language: English | Size: 1011.36 MB | Duration: 2h 27m
Explore the properties of amino acids, protein folding, purification and their structure determination techniques
What you'll learn
Understand the properties of amino acids and their functions.
Know the principles of different protein purification techniques.
Learn about secondary and super-secondary structures
Get an understanding of the principle and working of different protein structure determination techniques
Learn the theories of protein folding and their experimental validation.
To enroll in this course, all you need is some basic knowledge in chemistry.
Proteins are the workhorses inside the cell that carryout various functions such as metabolic reactions, provide structure to cells, transport oxygen, antibodies that fight infections, hormones and much more. The main building blocks of proteins are amino acids and they constitute the primary sequence information of the protein. Understanding the properties of amino acids and how they are arranged in different types of proteins can tell us a lot about the function of the protein.In this course you'll learn about1. The 3 types of classification of amino acids2. Learn how Ramachandran plot tells us about amino acid phi and psi angles that are involved in protein folding3. Learn the step-by-step process of protein sequencing4. Gain an understanding of the protein purification strategies5. How NMR and X-ray crystallography techniques are used to study the 3D structure of proteinsThis course contains has more than 2 hours of video content with 26 lectures explaining concepts pertaining to amino acids and proteins.By the end of the course, you'll be able to· write the 3 and 1 letter code of amino acids,· Identify R group of different amino acids· Describe secondary and super secondary structures formed by proteins· Calculate pH, pKa and pI values of amino acids· Summarize the operating principles of X ray crystallography, NMR and circular dichroism· Explain the principles of different protein purification strategies· Describe protein folding theories that best explain protein folding inside the cells.This course also contains bonus pdf files of content and a quiz towards the end of the lecture series to reinforce your learning. Anyone who wishes explore more about amino acids and proteins will benefit from this course.This course is intended for purchase as an adult. Those under 18 may use this course only if a parent or guardian opens their account, handles any enrollments, and manages their account usage.
Section 1: Introduction
Lecture 1 Introduction
Section 2: Amino acid properites
Lecture 2 Basic properties
Lecture 3 Classification
Lecture 4 Modified amino acids
Lecture 5 Beta amino acids
Lecture 6 Information storage
Lecture 7 Ionization properties
Section 3: Secondary structure
Lecture 8 Ramachandran plot
Lecture 9 Secondary structures formed by amino acids
Lecture 10 Other secondary structures
Lecture 11 Super secondary structures
Section 4: Sequencing
Lecture 12 Protein sequencing- Edman degradation
Section 5: Protein Folding
Lecture 13 An analogy
Lecture 14 Levinthal's paradox
Lecture 15 Anfinsen's experiment
Lecture 16 Folding theories
Lecture 17 Experiments to determine folding
Lecture 18 Protein stability
Section 6: Protein purification
Lecture 19 Based on Solubility
Lecture 20 Based on Molecular weight
Lecture 21 Based on pI
Lecture 22 Based on specific activity
Lecture 23 Protein Yield
Section 7: Protein structure determination techniques
Lecture 24 Circular Dichroism
Lecture 25 Nuclear Magnetic resonance
Lecture 26 X ray crystallography
Section 8: Wrap Up
Lecture 27 Summary
Lecture 28 BONUS
This course is designed for college or high school students who want to learn the basics of protein biochemistry,This course is for those students who want to refresh their concepts on amino acids and proteins.
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